Question

The chromatographic method of separating biochemical mixture of compounds, based on highly specific biological interactions is referred to as ______________

(a) thin layer chromatography

(b) ion-exchange chromatography

(c) affinity chromatography

(d) gel permeation chromatography

The question was posed to me in examination.

Query is from Enzymes Purification in division Enzymes Purification of Enzyme Technology

Answer 1

The correct answer is (c) affinity chromatography

To elaborate: Affinity chromatography is a method based on separation of molecules based on specific biological interaction such as antigen-antibody, enzyme-substrate etc. Thin layer chromatography is a technique which separates molecule based on their solubility in stationary or mobile phase. Ion-exchange chromatography is a technique based on separation of molecules using the ions present in them. Gel permeation chromatography is based on separation of molecules based on their size.