Question

The gradient separates the analyte mixture as a function of affinity of the analyte for the mobile phase relative to stationary phase.

(a) True

(b) False

This question was posed to me in an online interview.

This interesting question is from Enzymes Purification in division Enzymes Purification of Enzyme Technology

Answer 1

Correct answer is (a) True

For explanation: If the analytes have stronger interactions with mobile phase as compared to stationary phase, the analytes will elute faster and have shorter retention time. Whereas the analytes with stronger interactions with stationary phase as compared to mobile phase, will elute the analytes at later point of time and have longer retention time. Hence the above statement is true.