Question

Acetylcholine esterase can be purified by using affinity chromatography with the use of _______ as a ligand.

(a) ATP

(b) NADP

(c) Acrdinium

(d) Con A

The question was asked in unit test.

I need to ask this question from Enzymes Purification topic in section Enzymes Purification of Enzyme Technology

Answer 1

Correct choice is (c) Acrdinium

To elaborate: For acetylcholine esterase, acrdinium can be used as ligand. ATP can be used as ligand to purify adenosine kinase. Alcohol dehydrogenase is purified by using NADP as a ligand. Con A and mannose is used to purify acid phosphatase.