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Alkaline protease is purified by using ________

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Alkaline protease is purified by using ________

(a) DEAE-cellulose

(b) Q-sepharose

(c) Amberlite CG-50

(d) Sephacryl S-300

This question was posed to me during an online interview.

My question is based upon Enzymes Purification in portion Enzymes Purification of Enzyme Technology

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Correct option is (a) DEAE-cellulose

For explanation I would say: Alkaline protease is purified by using DEAE-cellulose which has been equilibrate with 10 mM Tris/ HCl buffer, containing 50 mM KCl. Q-sepharose is used to purify peroxidases. Amberlite CG-50 is used to purify cytochrome c. Sephacryl S-300 is used to purify alpha amylase by the gel permeation technique.

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