Ask a Question

Which one of the following cannot be used as alternative to ammonium sulphate?

← Prev Question Next Question →
+1 vote
in Enzyme Technology by (55.0k points)
Which one of the following cannot be used as alternative to ammonium sulphate?

(a) Lysozyme

(b) Methanol

(c) Acetone

(d) Propan-2-ol

I had been asked this question during an online interview.

My doubt is from Enzymes Purification topic in chapter Enzymes Purification of Enzyme Technology

1 Answer

+1 vote
by (727k points)
selected by
 
Best answer
Correct answer is (a) Lysozyme

To elaborate: Lysozyme is an enzyme which catalyzes the linkage between NAG and NAM of bacterial peptidoglycan. Hence it cannot be used as alternative to ammonium sulphate. Methanol, acetone and propan-2-ol can be used as alternative as they reduce the solubility of the enzyme by reducing dielectric constant of the medium.

Related questions

+1 vote
1 answer
Pick odd one out. (a)Histidine
asked Jan 29, 2022 in Enzyme Technology by vokoy (55.0k points)
+1 vote
1 answer
Pick odd one out. (a)Silica gel
asked Jan 29, 2022 in Enzyme Technology by vokoy (55.0k points)
We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

Powered by TalkJarvis
...