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To alter the retention time, which of the following parameters need not be manipulated?

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in Enzyme Technology by (55.0k points)
To alter the retention time, which of the following parameters need not be manipulated?

(a) Choice of column

(b) Choice of mobile phase

(c) Choice in flow rate

(d) Sample type

I have been asked this question in unit test.

Question is from Enzymes Purification topic in portion Enzymes Purification of Enzyme Technology

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The correct choice is (d) Sample type

Explanation: Identification of a compound by using HPLC is done by using a detector. The detector identifies in the form of peaks. The identifying peak should have a reasonable retention time and should be well separated from other extraneous peaks at detection level. To alter the retention time, the parameters which can be manipulated are choice of column, mobile phase and flow rate. Sample type does not matter.

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