Question

When the solvent phase in TLC is 20:80 mixture of hexane and ethyl acetate, the polar compounds will not move as much as non-polar compounds.

(a) True

(b) False

The question was asked in an interview.

Question is from Enzymes Purification topic in section Enzymes Purification of Enzyme Technology

Answer 1

Correct answer is (b) False

To elaborate: In TLC, when the mixture 20:80 mixture of hexane and ethyl acetate, the solvent will become mostly polar. This means polar compounds will move further up in the plate as compared to non-polar compounds. Hence the above statement is false.