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In the below diagram of organic solvent fractionation, what does Y represent?

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In the below diagram of organic solvent fractionation, what does Y represent?

(a) Stirring motor

(b) Thermometer

(c) Protein solution

(d) Dry-ice-ethanol water mixture

The question was posed to me in examination.

Asked question is from Enzymes Purification in chapter Enzymes Purification of Enzyme Technology

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Right answer is (d) Dry-ice-ethanol water mixture

To explain I would say: The above diagram represents the apparatus for organic solvent fractionation. Organic solvent fractionation utilizes dry ice-ethanol-water mixture for purification if desired protein. With constant addition of ethanol temperature is maintained as required. The apparatus below shows that Y represent dry-ice-ethanol-water mixture.

Hence Y represents dry-ice-ethanol-water mixture.

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