Ask a Question

Which of the following is a disadvantage of organic solvent fractionation?

← Prev Question Next Question →
+2 votes
in Enzyme Technology by (55.0k points)
Which of the following is a disadvantage of organic solvent fractionation?

(a) Faster than column techniques

(b) Denaturation of proteins

(c) Low density than salt solutions

(d) Short centrifugation times

The question was posed to me in an interview.

Question is taken from Enzymes Purification topic in portion Enzymes Purification of Enzyme Technology

1 Answer

+2 votes
by (727k points)
selected by
 
Best answer
The correct choice is (b) Denaturation of proteins

To explain: The major disadvantage of organic solvent fractionation is denaturation proteins due to use of organic solvent. Advantages are faster run time when compared to other column techniques. When compared to ammonium sulphate precipitation, it offer lower density to salt solutions and short centrifugation times to collect the precipitated enzyme.

Related questions

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

Powered by TalkJarvis
...