Correct choice is (c) Thin layer chromatography (TLC)
To explain I would say: In TLC, the stationary phase is immobilized on a glass plate and organic solvent act as the mobile phase. The sample dissolved in the solvent is placed on the stationary phase. The solvent mixture moves up by the capillary action and meet the sample mixture. Different compounds travel at different rates due to their solubility and interactions with the stationary phase absorbents. Gel exclusion, affinity and ion-exchange chromatography are used for purification of enzymes based on the property of the enzyme.