Question

In HPLC, the compounds are eluted using a constant mobile phase composition and is referred to as _____________

(a) nonspecific elution

(b) isocratic elution

(c) specific elution

(d) gradient elution

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I would like to ask this question from Enzymes Purification topic in chapter Enzymes Purification of Enzyme Technology

Answer 1

The correct answer is (b) isocratic elution

The best explanation: In isocratic elution, the compounds are eluted using a constant mobile phase composition. As compared to gradient elution in which the compounds are eluted using increasing strength of the organic solvent. Specific and nonspecific elution occurs during affinity chromatography. As compared gradient and isocratic elution which is taking place during HPLC.