Question

Alkaline protease is purified by gel filtration using __________ as gel filtration media.

(a) Sephacryl S-300

(b) Biogel – P

(c) Sephadex G-100

(d) Agarose gel

I have been asked this question in homework.

My question is based upon Enzymes Purification in section Enzymes Purification of Enzyme Technology

Answer 1

Right option is (c) Sephadex G-100

Easiest explanation: Crude alkaline protease is subjected to ammonium sulphate precipitation followed by dialysis against 0.1 M Tris-HCl. This sample is then applied on the sephadex G-100 gel equilibrated with 0.1 M Tris-HCl and eluted with the same buffer. Sephacryl S-300 is used to purify alpha amylase. Biogel – P and agarose are other commonly used gels for gel filtration.