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Peroxidases can be purified using ___________

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Peroxidases can be purified using ___________

(a) gel filtration

(b) cation exchanger

(c) anion exchanger

(d) affinity chromatography

This question was addressed to me during an internship interview.

Asked question is from Enzymes Purification topic in chapter Enzymes Purification of Enzyme Technology

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Right choice is (c) anion exchanger

Explanation: Peroxidases can be purified using anion exchanger which has Q-sepharose column and the sample is eluted with NaCl gradient of 0.35, 0.4, 0.45 and 0.5 M in Tris-HCl buffer. Cytochrome c is purified by using cation exchanger. Alpha amylase is purified by using gel filtration technique. Acetylcholine esterase is purified by using affinity chromatography.

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