Question

The chromatography technique in which the stationary phase may be solid or liquid is referred to as ________________

(a) ion-exchange chromatography

(b) thin layer chromatography (TLC)

(c) high performance liquid chromatography (HPLC)

(d) fast protein liquid chromatography (FPLC)

This question was posed to me in an internship interview.

The origin of the question is Enzymes Purification topic in section Enzymes Purification of Enzyme Technology

Answer 1

The correct choice is (d) fast protein liquid chromatography (FPLC)

Easiest explanation: In FPLC, the stationary phase is a solid or liquid in the form of matrix. In TLC, the stationary phase is immobilized on a glass plate and organic solvent acts as mobile phase. In HPLC, the sample is forced into the column at high pressure. Ion-exchange chromatography refers to use of ions for separation of the molecules.