Question

A preferable technique to perform fractionation is to cool the enzyme solution.

(a) True

(b) False

I have been asked this question in an online quiz.

This interesting question is from Enzymes Purification topic in division Enzymes Purification of Enzyme Technology

Answer 1

Correct choice is (a) True

Easy explanation: To avoid denaturation due to use of organic solvent, low temperatures need to be maintained. This can be done by using cold rooms at low fixed temperatures. But still it is difficult to maintain the enzyme at low temperatures during fractionation. Hence a preferred technique is to cool the enzyme solution. This can be done by either mechanically refrigerated bath or ice bath containing dry ice – ethanol-water mixture.