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_______________ is a liquid column chromatographic method of separating solute molecules according to differences in molecular size.

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_______________ is a liquid column chromatographic method of separating solute molecules according to differences in molecular size.

(a) Thin layer chromatography (TLC)

(b) Ion exchange chromatography

(c) Gel filtration

(d) Affinity chromatography

I got this question during an interview.

My enquiry is from Enzymes Purification in section Enzymes Purification of Enzyme Technology

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Right answer is (c) Gel filtration

To explain: The liquid chromatographic method of separating solute molecules according to differences in molecular size is referred to as gel filtration. TLC is a type of chromatography wherein the stationary phase is immobilized on a glass or plastic plate and organic solvent acts as the mobile phase. Ion-exchange and affinity chromatography are used in enzyme preparation according to the property of the enzyme.

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