Question

_____________ retains analyte molecules based on the coulombic interaction.

(a) Thin layer chromatography

(b) Affinity chromatography

(c) Gel filtration

(d) Ion exchange chromatography

The question was posed to me in examination.

I want to ask this question from Enzymes Purification topic in section Enzymes Purification of Enzyme Technology

Answer 1

The correct option is (d) Ion exchange chromatography

For explanation: Ion-exchange chromatography retains analyte molecules based on the coulombic or ionic interaction. Gel filtration retains analyte based on the size of the molecule. Thin layer chromatography retains analyte based on the solubility of the molecules in stationary or mobile phase. Affinity chromatography retains analyte based on the affinities between 2 molecules. For example, enzyme-substrate.