Question

_____________ can be achieved using HPLC by utilizing the fact that certain compounds have different migration rates given a particular column and mobile phase.

(a) Purification of a compound

(b) Chemical separations

(c) Identification of a compound

(d) Quantification of compound

This question was posed to me in homework.

Asked question is from Enzymes Purification in chapter Enzymes Purification of Enzyme Technology

Answer 1

The correct choice is (b) Chemical separations

The best I can explain: Chemical separations can be achieved by using HPLC. Separations can happen because of certain compound have different migration rate given a particular column and mobile phase. The extent of separation is mostly determined by the choice of stationary and mobile phase. The separation of target molecules from other compounds or contaminants is referred to as purification. Identification can be done by using HPLC with a detector. Quantification by using HPLC is determining the unknown concentration in solution.