Question

The chromatography in which the sample is forced through a column of the stationary phase by pumping a liquid (mobile phase) at high pressure through the column is referred to as ___________

(a) affinity chromatography

(b) fast protein liquid chromatography (FPLC)

(c) thin layer chromatography (TLC)

(d) high performance liquid chromatography (HPLC)

This question was posed to me in examination.

This key question is from Enzymes Purification topic in division Enzymes Purification of Enzyme Technology

Answer 1

Correct option is (d) high performance liquid chromatography (HPLC)

To explain I would say: The basic principle of HPLC is that the sample is forced through a column of the stationary phase by pumping a liquid at high pressure through the column. In TLC, there is a stationary phase immobilized on a glass plate and an organic solvent that acts as a mobile phase. Affinity chromatography is a separation technique based on the binding affinities between two molecules. FPLC is a technique in which the stationary phase is liquid or solid, in the form of a matrix.