Question

Homogenization performed by passing the cells at high pressure through narrow orifice which can also cause cell rupture is referred to as _______________

(a) ultrasonicator

(b) freezer

(c) mortar and pestle

(d) french press

I had been asked this question by my school teacher while I was bunking the class.

Origin of the question is Extraction of Enzymes by Physical Methods topic in portion Enzyme Extraction and Isolation of Enzyme Technology

Answer 1

Right choice is (a) ultrasonicator

Explanation: French press is one which the cells are forced at high pressure through narrow orifice is referred to as french press. This procedure also causes the cell rupture. Freezer is refrigerator with temperatures as low as -20° C or -80° C. Ultrasonicator uses ultrasonic waves to lyse the cells. Mortar and pestle is a traditional homogenizer.