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A _________ has long teflon tube about 30-40 cm long which is bulged at the end and has an automated machine for providing the pressure.

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A _________ has long teflon tube about 30-40 cm long which is bulged at the end and has an automated machine for providing the pressure.

(a) ultrasonicator

(b) homogenizer

(c) osmotic shock

(d) mortar and pestle

The question was asked in class test.

This intriguing question originated from Extraction of Enzymes by Physical Methods in portion Enzyme Extraction and Isolation of Enzyme Technology

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Right option is (c) osmotic shock

Easiest explanation: Mortar and pestle is a traditional homogenization method where manual pressure is applied. As compared to a modern homogenizer wherein automated pressure is applied through a long teflon tube about 30-40 cm long which is bulged at the end. Ultrasonicator is used to perform ultrasonication on cells to perform cell lysis. Osmotic shock is chemical method for cell lysis which utilizes 1.4% buffered sucrose. It applies the technology of hypertonic and hypotonic solution.

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