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Which of the following is not a physical method for extraction of enzymes?

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in Enzyme Technology by (55.0k points)
Which of the following is not a physical method for extraction of enzymes?

(a) Osmotic shock

(b) Ultrasonication

(c) Homogenization

(d) Freezing technique

This question was posed to me in an online interview.

The origin of the question is Extraction of Enzymes by Physical Methods topic in chapter Enzyme Extraction and Isolation of Enzyme Technology

1 Answer

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Best answer
Right choice is (a) Osmotic shock

Explanation: Osmotic shock is a chemical method for extracting enzymes by using 1.4% buffered sucrose. Osmotic shock is a technique which adopts the hypertonic or hypotonic condition for cell lysis. For extracting enzymes, physical methods include ultrasonication, homogenization, freezing technique, etc.

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