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Which of the following should be implemented in case of extracting conjugating enzymes?

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Which of the following should be implemented in case of extracting conjugating enzymes?

(a) Apt chemical compounds

(b) Suitable density gradient

(c) Centrifugation technique

(d) Proper filtration

I got this question during an online interview.

Question is from Enzyme Extraction and Isolation topic in section Enzyme Extraction and Isolation of Enzyme Technology

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Right answer is (b) Suitable density gradient

The best explanation: Conjugating enzymes are intracellular enzymes that are located in liver mitochondria. In order to avoid contamination from other proteins, we need to use a suitable density gradient which will remove unwanted debris. Apt chemical compounds, centrifugation and proper filtration can be used in case extracting extracellular enzyme as it is much easier to isolate.

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