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Which of the following methods cannot be used to isolate enzymes from cell free extract?

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in Enzyme Technology by (55.0k points)
Which of the following methods cannot be used to isolate enzymes from cell free extract?

(a) Use of factors like pH and ionic strength

(b) Use of chemicals

(c) Homogenization

(d) Use of temperature factor

I got this question in unit test.

The doubt is from Isolation of Enzymes topic in portion Enzyme Extraction and Isolation of Enzyme Technology

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Right option is (c) Homogenization

The explanation is: Nucleic acid is the major contaminant in the cell free extract along with lipids, simple sugars and so on. Factors like temperature, pH and ionic strength can be used to remove the contaminants and isolate enzymes. Chemicals such as streptomycin sulphate, nucleases etc., can be used to degrade nucleic acids and isolate the required enzymes.

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