Question

The increase in pH affects the inter H2 bonds and intramolecular hydrophobic bonds are majorly affected leading to denaturation of DNA.

(a) True

(b) False

The question was posed to me in unit test.

This question is from Isolation of Enzymes in chapter Enzyme Extraction and Isolation of Enzyme Technology

Answer 1

Correct option is (b) False

Easiest explanation: Increase or decrease in pH leads to protonation and deprotonation of bases which leads to denaturation of DNA. In this case, the inter H2 bonds and intramolecular hydrophobic bonds are majorly affected to due to increase in temperature and not pH. This leads to denaturation of DNA and isolating the required enzyme. The main limitation in this case is denaturation of enzymes also, which can be prevented by optimizing the temperature and time parameters.