Question

In ___________ treatment, certain molecules interact through ionic or Vander wall forces which leads to solubilization of phospholipids and lipoprotein which in turn, increases membrane permeability directing to cell lysis.

(a) Lysozyme

(b) EDTA

(c) Detergent

(d) NaoH

The question was asked by my college director while I was bunking the class.

The query is from Extraction of Enzymes by Chemical Methods topic in chapter Enzyme Extraction and Isolation of Enzyme Technology

Answer 1

Right choice is (c) Detergent

The explanation: In detergent treatment, Cell lysis is performed by increasing membrane permeability by solubilizing phospholipids and lipoproteins present in the cell wall. This is done through interaction if detergents through ionic or Vander wall forces. In lysozyme treatment, lysozymes act by catalyzing the β (1 → 4) glycosidic linkages of NAM and NAG present in the cell wall. EDTA acts as a divalent chelator and chelate ions present in the cell wall. NaOH is an alkali and act by having a nucleophilic attack on the cell wall or cell membrane.