Question

Alkali treatment is not advisable for which of the following?

(a) Extracellular enzymes

(b) Bacterial enzymes in cell wall

(c) Enzymes in cell wall of plant sources

(d) Membrane bound enzymes in eukaryotic cell

I got this question by my school teacher while I was bunking the class.

This interesting question is from Extraction of Enzymes by Chemical Methods in portion Enzyme Extraction and Isolation of Enzyme Technology

Answer 1

Correct option is (a) Extracellular enzymes

To explain I would say: Alkali treatment is used extracting enzymes from bacterial and plant cell wall. It is also useful in extracting membrane bound enzymes in eukaryotic cell. But extracellular enzymes are difficult to extract from this method. Hence it is not advisable to use alkali treatment for extraction of extracellular enzymes.