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__________ is a method which is highly used for lab scale procedures than large scale work.

(a) Ultrasonication

(b) Homogenization

(c) Freezing

(d) Alkali treatment

I got this question by my college professor while I was bunking the class.

The above asked question is from Extraction of Enzymes by Physical Methods in division Enzyme Extraction and Isolation of Enzyme Technology

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Right option is (a) Ultrasonication

To elaborate: Ultrasonication is a method which uses ultrasonic waves to lyse the cells. Shock waves are formed due to collapses in area of rarefaction. Hence this method is used for lab scale procedures as compared to large scale work. Homogenization is widely used to disrupt the cell wall or cell membrane. Freezing is nothing but cold or psychrophilic shock. Alkali treatment is a chemical method that uses alkali to lyse the cells.

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