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__________ is a compound which is frequently used for DNA removal.

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__________ is a compound which is frequently used for DNA removal.

(a) 1.4% buffered sucrose

(b) Protamine sulphate

(c) Sodium hydroxide

(d) Lysozyme

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This interesting question is from Isolation of Enzymes in division Enzyme Extraction and Isolation of Enzyme Technology

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Right choice is (b) Protamine sulphate

To explain I would say: Protamine sulphate is a compound which is frequently used for DNA removal, inturn isolating the enzyme of interest. 1.4% buffered sucrose is used for osmotic shock which is a chemical method for extracting the enzyme from cells. Sodium hydroxide is used in the alkali treatment which brings about nucleophilic attack and releasing the cell contents. Lysozyme is used to extract enzymes from gram positive bacteria as they have high content of peptidoglycan. As lysozyme catalyzes β(1 → 4) glycosidic linkage between NAG and NAM present in bacterial peptidoglycan.

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