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Homogenization utilizing ____________ is one where in manual pressure is created for cell lysis.

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Homogenization utilizing ____________ is one where in manual pressure is created for cell lysis.

(a) mortar and pestle

(b) ultrasonicator

(c) freezer

(d) french press

The question was posed to me in exam.

This interesting question is from Extraction of Enzymes by Physical Methods topic in section Enzyme Extraction and Isolation of Enzyme Technology

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Correct option is (a) mortar and pestle

Easiest explanation: Traditional method for homogenization utilizes mortar and pestle wherein manual pressure is created by pestle in a mortar for cell lysis. Ultrasonicator is a machine used for ultrasonication. Freezer is refrigerator with temperatures as low as -20° C or -80° C. French press is one which the cell are ruptured by passing through small orifice.

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