Question

Which one if the chemicals is not used to isolate enzymes from cell free extract?

(a) 1.4% buffered sucrose

(b) Cetyl trimethyl ammonium bromide

(c) Nucleases

(d) Streptomycin sulphate

This question was addressed to me during an internship interview.

I'm obligated to ask this question of Isolation of Enzymes in portion Enzyme Extraction and Isolation of Enzyme Technology

Answer 1

The correct choice is (a) 1.4% buffered sucrose

Best explanation: 1.4% buffered sucrose is the chemical used during osmotic shock which is a chemical method to extract enzymes from cells. Whereas cetyl trimethyl ammonium bromide, nucleases, streptomycin sulphate, etc., are the chemicals used to isolate enzymes from cell free extract which has nucleic acids as the main contaminant along with lipids, simple sugar and so on.