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Which of the following is not a technique for the extraction of enzymes by chemical methods?

(a) Alkali treatment

(b) Osmotic shock

(c) Detergent treatment

(d) Homogenization

The question was posed to me during an interview.

My question is from Extraction of Enzymes by Chemical Methods topic in division Enzyme Extraction and Isolation of Enzyme Technology

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Right choice is (d) Homogenization

Explanation: Homogenization is a method to extract enzymes using physical methods. Chemical methods involve different chemicals like EDTA, lysozymes, NaoH etc. to isolate enzymes from selected sources. Hence alkali treatment, Osmotic shock and Detergent treatment falling into the category extracting enzymes by chemical methods.

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