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____________ is a phenomenon of assuming frequencies more than 20 kHz which is above the human audibility.

(a) Homogenization

(b) Ultrasonics

(c) Alkali treatment

(d) Freezing

The question was posed to me in an interview for job.

The above asked question is from Extraction of Enzymes by Physical Methods in portion Enzyme Extraction and Isolation of Enzyme Technology

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Right option is (b) Ultrasonics

The best I can explain: The phenomenon which assumes frequencies more than 20 kHz. This frequency is above human audibility which corresponds to 6.1 to 2.3 *10^-4 cm wavelength. Homogenization is a mechanical technique adopted widely to disrupt the cell wall and cell membrane. Freezing is nothing but a cold or psychrophilic shock. Alkali treatment is a chemical method for extraction of enzymes which uses a nucleophilic attack of basic radicals on the cell wall.

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