Question

____________ technique is nothing but a cold or psychrophilic shock.

(a) Ultrasonication

(b) Homogenization

(c) Freezing

(d) Alkali treatment

This question was addressed to me by my school principal while I was bunking the class.

My doubt is from Extraction of Enzymes by Physical Methods topic in portion Enzyme Extraction and Isolation of Enzyme Technology

Answer 1

Right choice is (c) Freezing

Best explanation: Freezing technique is one which cells are subjected to cold temperatures as low as minus degree Celsius. Due to which the cells get a cold or psychrophilic shock which leads to cell lysis. Ultrasonication uses shock waves whereas homogenization uses mechanical pressure to lyse the cells. Alkali treatment is a chemical method to lyse the cells because of nucleophilic attack on cell walls by the basic radicals.