Question

If the solvent phase in TLC was 80:20 mixture of hexane and ethyl acetate, the polar compounds will not move as much as non-polar compounds.

(a) True

(b) False

This question was posed to me in my homework.

Query is from Enzymes Purification in portion Enzymes Purification of Enzyme Technology

Answer 1

Correct option is (a) True

The explanation is: When the solvent is 80:20 mixture of hexane and ethyl acetate, the solvent will be mostly nonpolar. This means that the non-polar compounds will move further up the plate. In contrast the polar compounds will not have moved as much as the non-polar compounds. Hence the statement is true.