Question

The process in which there is a free flow low molecular weight compounds across the membrane retaining high molecular weight compound is referred to as _____________

(a) purity checking

(b) dialysis

(c) salting out

(d) precipitation

I had been asked this question in an international level competition.

I want to ask this question from Enzymes Purification topic in chapter Enzymes Purification of Enzyme Technology

Answer 1

Correct choice is (b) dialysis

Easy explanation: Purity checking and precipitation are the steps involved in purification. Salting out is a phenomenon to precipitate the enzyme by reducing its solubility by reducing the available water content. Dialysis uses a membrane to allow free flow of low molecular weight compounds retaining the high molecular weight compounds inside.