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In _________ elution, the compounds are eluted by increasing the strength of the organic solvent during HPLC.

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In _________ elution, the compounds are eluted by increasing the strength of the organic solvent during HPLC.

(a) nonspecific

(b) gradient

(c) specific

(d) isocratic

I have been asked this question in homework.

The origin of the question is Enzymes Purification topic in division Enzymes Purification of Enzyme Technology

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The correct option is (b) gradient

The best I can explain: Specific and nonspecific elution occurs during affinity chromatography. Isocratic elution is one which the elution is done using a constant mobile phase. The process of eluting compounds during HPLC by increasing the strength of the organic solvent is referred to as gradient HPLC. The sample is injected on weaker mobile phase and the strength is increased in linear fashion by raising the organic solvent fraction which results in the elution of retained compounds.

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