Question

Which among these is not a way of non-specific elution?

(a) Solvent change

(b) pH change

(c) Reversible denaturation

(d) Competition to suitable complementary protein

This question was posed to me by my college director while I was bunking the class.

My question is from Enzymes Purification in portion Enzymes Purification of Enzyme Technology

Answer 1

Right answer is (d) Competition to suitable complementary protein

To elaborate: Nonspecific elution can be done by using solvent or buffer changes, pH or ionic strength changes, the application of chaotropic reagents, temperature changes, reversible denaturation and destruction of spacer arm. Competition to suitable complementary protein is a type of specific elution.