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Which of the following is not a highly specific biological interaction to be used in affinity chromatography?

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+1 vote
in Enzyme Technology by (55.0k points)
Which of the following is not a highly specific biological interaction to be used in affinity chromatography?

(a) Cations-anions

(b) Antigen-antibody

(c) Enzyme-substrate

(d) Receptor-ligand

This question was addressed to me by my school teacher while I was bunking the class.

This is a very interesting question from Enzymes Purification in chapter Enzymes Purification of Enzyme Technology

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Right choice is (a) Cations-anions

For explanation I would say: Cations-anions are used in ion-exchange chromatography to separate molecules based on the ions present in them. Antigen-antibody, enzyme-substrate, receptor-ligand are used in affinity chromatography to separate highly specific biological interactions molecules.

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