Question

Cation exchange resin is used to purify __________

(a) alkaline protease

(b) peroxidase

(c) alpha amylase

(d) cytochrome c

This question was posed to me by my college professor while I was bunking the class.

This interesting question is from Enzymes Purification topic in portion Enzymes Purification of Enzyme Technology

Answer 1

The correct answer is (d) cytochrome c

Easiest explanation: Cation exchange resin prepared using amberlite CG-50 is used to purify cytochrome c. Alkaline protease and peroxidase are purified by using anion exchangers. Alpha amylase is purified by using a gel filtration technique using Sephacryl S-300.