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Which characteristic of the enzyme can be used for purifying it by using FPLC?

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Which characteristic of the enzyme can be used for purifying it by using FPLC?

(a) Molecular weight

(b) Enzyme-substrate

(c) Electric charge

(d) Hydrophobicity

I have been asked this question by my college director while I was bunking the class.

The above asked question is from Enzymes Purification in chapter Enzymes Purification of Enzyme Technology

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Right answer is (b) Enzyme-substrate

The best explanation: Enzyme substrate interaction is utilized while performing affinity chromatography. Enzyme possessing various characteristic such as molecular weight, electric charge and hydrophobicity can be purified by adopting FPLC.

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