Question

Ammonium sulphate is convenient and effective chemical for fractionation by salting out method.

(a) True

(b) False

The question was asked in an online quiz.

My doubt stems from Enzymes Purification topic in section Enzymes Purification of Enzyme Technology

Answer 1

The correct answer is:

(a) True

Ammonium sulfate is indeed a convenient and effective chemical used in the salting out method for the fractionation and purification of proteins, including enzymes. This method works by increasing the ionic strength of a solution, which causes proteins to precipitate out of solution based on their solubility, helping to separate different proteins from each other. Ammonium sulfate is commonly used because it is highly soluble and allows for a gradual and controlled precipitation process.