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Which gel filtration media is used to purify alpha amylase by gel filtration?

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Which gel filtration media is used to purify alpha amylase by gel filtration?

(a) Biogel – P

(b) Sephacryl S-300

(c) Biogel – A

(d) Sephadex

I got this question in an online interview.

I need to ask this question from Enzymes Purification topic in section Enzymes Purification of Enzyme Technology

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Best answer
Correct choice is (b) Sephacryl S-300

The best explanation: Sephacryl S-300 is used as the gel filtration media to purify alpha amylase by gel filtration. The column is equilibrated with NaCl and the sample of interest in eluted with NaCl of different concentration. Others commonly used gel filtration media are biogel – P, biogel – A and sephadex.

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