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Acid phosphatase: Mannose:: Alpha amylase: ______________

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Acid phosphatase: Mannose:: Alpha amylase: ______________

(a) KCl

(b) Glycogen

(c) Adenosine

(d) Methyl-D-mannoside

I have been asked this question in an online interview.

The origin of the question is Enzymes Purification in division Enzymes Purification of Enzyme Technology

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Best answer
Correct choice is (b) Glycogen

For explanation: For purifying acid phosphatase, the eluent used is Mannose. In the same way, alpha amylase can be purified by using glycogen as an eluent. Adenosine is used as an eluent for purifying adenosine kinase and methyl-D-mannoside is used as an eluent for acetylcholine esterase.

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