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In affinity chromatography, the matrix should be chemically and mechanically stable.

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in Enzyme Technology by (55.0k points)
In affinity chromatography, the matrix should be chemically and mechanically stable.

(a) True

(b) False

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I need to ask this question from Enzymes Purification topic in section Enzymes Purification of Enzyme Technology

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Right choice is (a) True

The explanation: In affinity chromatography, the matrix selected should be chemically and mechanically stable because of coupling conditions and varying conditions pH, ionic strength, temperature and presence of denaturants which may be needed for adsorption or elution. Due to this, it permits the repeated use of certain specific adsorbents. Hence the above statement is true.

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