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In which chromatographic technique, the solvent velocity and constant flow rate is controlled?

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in Enzyme Technology by (55.0k points)
In which chromatographic technique, the solvent velocity and constant flow rate is controlled?

(a) Affinity chromatography

(b) Ion-exchange chromatography

(c) Fast protein liquid chromatography (FPLC)

(d) Gel permeation

This question was addressed to me in an interview for internship.

The query is from Enzymes Purification in chapter Enzymes Purification of Enzyme Technology

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Best answer
Correct choice is (c) Fast protein liquid chromatography (FPLC)

Easiest explanation: During FPLC, the solvent velocity and constant flow rate is controlled by using pumps. Affinity chromatography is a technique which separate molecules based on the affinities between different molecules. Gel permeation allows separation based on the size of the separating molecule. Ion-exchange chromatography is a technique which utilizes ion for separation of molecules.

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