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The ratio of distance travelled by the sample over the total distance covered by the solvent is referred to as ___________

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The ratio of distance travelled by the sample over the total distance covered by the solvent is referred to as ___________

(a) solubility

(b) miachelis menton constant

(c) retention factor

(d) volumetric throughput

I had been asked this question in an interview for internship.

My question is taken from Enzymes Purification in chapter Enzymes Purification of Enzyme Technology

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Correct choice is (c) retention factor

Explanation: Retention factors refers to the ratio of distance travelled by the sample to the distance travelled by the solvent. Volumetric throughput comes into picture when dealing with filtration. This is proportional to pressure and filter area and inversely proportional to filter cake thickness and dynamic viscosity. Solubility comes into picture when dealing with ammonium sulphate precipitation and is given by Log S = Kintercept – Salt T.

Miachelis Menton constant deals with enzyme kinetics is given by Km which is nothing but the substrate concentration when Vmax is reduced to half.

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