Question

The process of determining the unknown concentration of a compound in a known solution by using HPLC is referred to as _______________

(a) quantification of a compound

(b) purification of a compound

(c) chemical separations

(d) identification of a compound

The question was asked in examination.

This interesting question is from Enzymes Purification topic in section Enzymes Purification of Enzyme Technology

Answer 1

Right option is (a) quantification of a compound

To explain I would say: Quantification by using HPLC refers to the process of determining the unknown concentration of a compound in a known solution. It involves injection of series of known concentration of the standard solution onto HPLC. This is detected as series of peaks that is correlated to concentration of compound injected. By comparing the peak of unknown concentration these known peaks, the concentration can be quantified.