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In which of the following technique, the system is calibrated by using markers of known molecular weight to determine the unknown molecular weight?

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In which of the following technique, the system is calibrated by using markers of known molecular weight to determine the unknown molecular weight?

(a) Mass spectrometry

(b) Ultracentrifugation

(c) Gel filtration

(d) SDS-gel electrophoresis

I got this question during an interview.

I'm obligated to ask this question of Determination of Molecular Weight of Enzymes topic in portion Enzyme Extraction and Purification of Enzyme Technology

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The correct option is (d) SDS-gel electrophoresis

The best I can explain: In SDS-gel electrophoresis, the system is calibrated by using markers of known molecular weight to determine the unknown molecular weight. Whereas in gel filtration the column is calibrated with known molecular weight proteins and the elution volume of each protein is found and, hence the unknown molecular weight is determined. Mass spectrometer is based on the charge to mass ratio, whereas ultracentrifugation is based on the sedimentation rate of spherical particles.

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