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Which of the following enzyme is assayed and measured using Spectrophotometer?

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Which of the following enzyme is assayed and measured using Spectrophotometer?

(a) Hydrolases

(b) Bacterial luciferase

(c) Phosphofructokinase

(d) Glutamate decarboxylase

This question was addressed to me by my school principal while I was bunking the class.

The origin of the question is Enzyme Assay Techniques in chapter Enzyme Assay & Enzyme Catalysis of Enzyme Technology

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Right answer is (c) Phosphofructokinase

For explanation: Phosphofructokinase is assayed and measured using spectrophotometer. This has been linked with aldolase to glyceraldehyde-3-phosphate dehydrogenase reaction. Hydrolases is measured by using spectrofluorometric method, whereas bacterial luciferase is measured by using luminescence method. Radioisotope method is used to measure by assaying enzyme glutamate decarboxylase.

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